Vectors for tagging the protein with a florescent protein fused to a double affinity tag
We developed several vectors for tagging the proteins with a modular tag. The tag contains one of the versions of a monomeric fluorescent protein (GFP (S65T), EYFP, mOrange, mCitrine, Cherry etc.) fused at the C-terminus to a small double affinity tag for immunopurification (3xFLAG-6xH, 3xMyc-8xH, 4Strep-8xH, etc). Here are the sequences of vectors we have:
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