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Eisosomes
We applied out methods and tools to investigate the composition and the dynamics of Eisosomes. We tagged two proteins Pil1 and Lsp1 with the our modular tags and, purified and identified several proteins. Using reciprocal tagging we confirmed that some of the identified proteins co-localize with Pil1 and Lsp1, which indicates that they could be essential components of Eisosomes.
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APC
We purified yeast APC and mapped phosphorylation sites on several subunits: Apc1, Cdc16, Cdc23, Cdc26 and Cdc 27 using a modular mass spectrometric tool. We now puriffy cell-cycle specific APC complexes to profile the composition and the abundances of the phosphorylation sites on the subunits during the different stages of the cell cycle.
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Nuclear periphery proteins.
We study protein composition and cell-cycle dynamics of a number of nuclear periphery proteins from yeast and human cells. We use cell-cycle sub-cellular localization and protein-protein interaction data as clues to uncover the processes, in which these proteins are involved.
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Discovery and characterization of new substrates of Separase.
We about to initiate a project with the aim to discover and characterize the new substrates of separase. The technique and tools we develop could be extremely helpful for achieving this goal.
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